ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Sejeong Kim1,2, Jiyoon Lee3, Soomin Lee1,2, Jimyeong Ha1,2, Jeeyeon Lee1,2, Yukyung Choi1,2, Hyemin Oh1,2, Yohan Yoon1,2 and Kyoung-Hee Choi4
1Department of Food and Nutrition, Sookmyung Women’s University, Seoul 04310, Korea.
2Risk Analysis Research Center, Seoul 04310, Korea.
3Center for Food and Bioconvergence, SNU, Seoul 08826, Korea.
4Department of Oral Microbiology, College of Dentistry, Wonkwang University, Iksan, Jeonbuk 54538, Korea.
J Pure Appl Microbiol, 2019, 13 (2): 647-652 | Article Number: 5568
Received: 11/04/2019 | Accepted: 06/06/2019 | Published: 29/06/2019
Abstract

Staphylococcus aureus is a known enterotoxin-producing foodborne pathogen; however, the invasion mechanism of the bacterium into intestinal cells remains unclear. The aim of this study was to determine whether S. aureus can invade Caco-2 cells, and to elucidate the gene responsible for this invasion. Caco-2 cells were infected with S. aureus strains NCCP10862, KACC13236, KACC10768 and KACC11596, and their invasion efficiencies were evaluated. Proteins found in the invasive and noninvasive S. aureus strains were labelled with isobaric tags for relative and absolute quantification (iTRAQ), and the gene encoding the protein responsible for S. aureus invasion was deleted using a temperature-sensitive plasmid, pIMAY. The Caco-2 cell invasion efficiencies of the wild type and mutant S. aureus were then compared. Among the S. aureus strains, only NCCP10862 and KACC10768 were able to invade Caco-2 cells, and these strains had a higher level of pyruvate formate lyase (Pfl) protein expression than that of the noninvasive strains. Therefore, a pflB-deletion mutant of KACC10768 was prepared, which revealed a 60% decrease in invasion efficiency when compared to the wild type. These results indicate that certain S. aureus strains can invade intestinal cells, and the protein encoded by the pfl gene is involved in this invasion.

Keywords

Staphylococcus aureus, invasion, pyruvate formate lyase, foodborne pathogen.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.