This study analysed Pseudomonas aeruginosa and Staphylococcus aureus burn wound infections, in order to evaluation their incidence, histological change and antimicrobial susceptibilities. Out of 39 burn wound cases admitted to surgery department, 20 and 12 strains of P. aeruginosa and S. aureus respectively were isolated from pyogenic burned skin lesions. Antibiotic resistance sketches of these strains to antibiotics were strategized. All the tested strains were multiple antibiotic resistance. Developed rates of susceptibility were confirmed for P. aeruginosa isolates against cefotoxim, gentamicin and nitrofurantoin. Also this study was to examine relation between the demonstrated antibiotic resistance and the occurrence of plasmids. Molecular sizes of the noticed plasmids were 24,321 kbp in P. aeruginosa and 23,25 kbp in S. aureus. Plasmid curing in grouping with MIC purpose revealed that resistance of P. aeruginosa and S. aureus isolates was plasmid allied. Histological analysis of burn wound infection is created on the thought of microorganisms attacking viable tissue below the eschar surface. The great MAR recognised marks it required for antibiotic resistance testing to be piloted former to antibiotics remedy for burn wound infection.