ISSN: 0973-7510

E-ISSN: 2581-690X

A. Mandal, S. Kar, P.K. Das Mohapatra, C. Maity, K.C. Mondal and B.R. Pati
Department of Microbiology, Vidyasagar University, Medinipur – 721 102, India.
J Pure Appl Microbiol. 2008;2(1):155-160
© The Author(s). 2008
Received: 12/12/2007 | Accepted: 25/01/2008 | Published: 30/04/2008
Abstract

Extracellular xylanase production by the newly isolated Bacillus cereus BSA1 was optimized under submerged fermentation. The growth of bacteria and its enzyme production were increased upto 72 h and after that bacteria turned to its stationary phase but enzyme production reached its maximum level at 84 h. Incubation temperature of 35oC and initial medium pH of 6.0 were found to be optimum for xylanase production. Pure xylan induced higher amount of xylanase production in respect to other tested agricultural wastes. Maximum enzyme production by the organism was obtained with a xylan concentration of 0.5% (w/v). The xylanase production became repressed when readily available monosaccharides like glucose, xylose, arabinose (0.5% w/v) were added to the xylan containing medium. This article sounds for the first time of smart and successful production of xylanase from a mesophilic soil bacterium, Bacillus cereus BSA1.

Keywords

Bacillus cereus, xylanase, optimization, submerged fermentation

Article Metrics

Article View: 739

Share This Article

© The Author(s) 2008. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.