Contaminants in air and water contribute largely to the occurrence of a number of diseases and health problems. TiO2 has been used as a photo-catalyst in various fields such as water purification, air-filters, disinfection and sterilization. The present study is focused on the synthesis of TiO2 and TiO2 doped nanofibers to achieve highly efficient air filters and to obtain increased surface area to volume ratio and optimize concentration of the Ag and Zn in the main matrix of TiO2 to get the better inhibition of both bacterial and fungal species. The synthesized nanofibers were further characterized by employing the standard techniques such as FTIR, SEM and X-RD for Chemical, Morphological and for Structural studies, respectively. The antibacterial of bacterial species: Bacillus subtilis (BS), Staphylococcus epidermidis (SE), Staphylococcus aureus (SA) and Staphylococcus faecalis (SF) and fungal species Candida tropicalis (CT), Candida parapsilosis (CP), Candida albicans (CA) and Candida glabrata (CG) were evaluated.