0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.13.4.09 Toxicity Test for the Extract of Symbiont Bacteria Bacillus sp. as Anti-bacteria Isworo Slamet Sulistyani Environmental Health Department, Faculty of Health Science, Dian Nuswantoro University, Semarang, Indonesia. Environmental Health Department, Faculty of Public Health, Diponegoro University, Semarang, Indonesia. 04 12 2019 13 4 1977 1984 Copyright © 2019 The Author(s) 2019 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.https://creativecommons.org/licenses/by/4.0/

Increased antibiotic resistance spurs exploration of bioactive compounds as new antibiotic alternatives. Bacillus sp. is a symbiont bacterium which is a marine microorganism that has the potential to produce new bioactive compounds that can be developed as new antibiotics. This research is an experimental study aimed at identifying bioactive compounds by thin layer chromatography methods and testing the activity of bioactive compounds by probit analysis EPA probit analysis program version 1.5. in Artemia salina Leach. Bioactive compounds identified were compounded from the alkaloid group with the category of highly toxic several-irritating base on the EPA toxicity category and the highly hazardous base on The WHO toxicity category based on the environmental protection agency probit analysis program used for calculating LC / EC value of LC 50 = 169,520 g / l. Symbiont Bacillus sp. produce secondary metabolites in the form of bioactive which have the ability as anti-bacteria. As a new antibiotic alternative to overcome resistance, especially in methicillin-resistant bacteria

 Keywords Bacillus sp., Artemia salina Leach secondary metabolites bioactive highly toxic several – irritating symbiont highly hazardous brine shrimp lethality toxicity