0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.13.3.63 Screening, Isolation and Identification of Thermophilic Esterase Enzyme Isolated from Rhodococcus SP: LKE-021 Singh Lekha Sharma Gaurav Awasthi Gyanendra Kumar Lokendra Irfan Ali Mohammad Moin Sarmad School of Applied Sciences, Suresh Gyan Vihar University, Jaipur – 302 033, India. Department of Biochemistry, Dolphin PG Institute of Biomedical and Natural Science, Dehradun – 248 007, India Department of Microbiology, PM College of Education Karsua, Aligarh – 202 001, India 30 09 2019 13 3 1855 1861 Copyright © 2019 The Author(s) 2019 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. https://creativecommons.org/licenses/by/4.0/

Rhodococcus sp. LKE-021 from soil samples of the region of Gangotri (10,000 feet of average height) of Uttarakhand Himalayas, India, produced a thermophilic esterase. The physiological and morphological characteristics of the isolated Rhodococcus sp. LKE-021 detected as Gram Positive, rod shape, catalase positive, indole negative, positive to glucose and xylose fermentation test, and can grow on the Nutrient Broth medium. Esterase production confirmed on the basis of spectrophotometric enzyme assay. Taxonomic characteristics Rhodococcus confirmed by 16s rRNA gene sequencing.

 Keywords Esterase hydrolytic enzymes Rhodococcus LKE -021 extremophiles enzyme thermophilic enzyme