Opportunistic pathogens prevail in the hospital environment, and utensils are the root cause of severe nosocomial infection. These pathogens exhibit high antibiotic resistance due to constant exposure to drug therapy. This study focuses on screening antibiotic-resistant opportunistic pathogen and effectiveness of piperidine compounds against the opportunistic pathogens. Standard microbiological laboratory protocols were used and followed, and about 238 samples were processed and screened. Among them, 47 reported positive for the presence of pathogens like Staphylococcus species, Salmonella species, Pseudomonas species, Proteus species, E. coli and Klebsiella species. In antibiotic resistance screening, the maximum resistance percentage was recorded against Ampicillin and Chloramphenicol (100%). The least percentage of resistance was noticed against Carbenicillin (41%). Piperidine compounds showed promising susceptibility towards test isolates. The MIC of the compounds against E. coli and Staphylococcus sp. was found to be higher when compared to Klebsiella sp.