0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.13.3.41 Production and Flocculating Performance of Bioflocculant by Bacterial Strain and its Application for Municipal Wastewater Treatment Vimala R.T.V Department of Biotechnology, Bharathidasan University, Tiruchirapalli – 620 024, Tamil Nadu, India. 19 09 2019 13 3 1673 1681 Copyright © 2019 The Author(s) 2019 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. https://creativecommons.org/licenses/by/4.0/

In the present research, bioflocculant was produced from Bacillus subtilis using molecular methods. The structural and functional features of the bioflocculant were determined that it consisted of 64% carbohydrate and 18% protein. Production medium for bioflocculant mainly comprised Sucrose 16 g; Peptone 2 g; MgSO4·7H2O 0.3 g were statistically optimized to increase the bioflocculant production. They were further characterized by Field emission scanning electron microscopy (FE-SEM), and Fourier-transform infrared spectroscopy (FTIR).Therefore, they could be considered as a suitable method for treating municipal wastewater based on high flocculating rate.

Keywords Bioflocculant Wastewater RSM Kaolin Treatment COD BOD