In recent days, antibiotic producing fluorescent pseudomonads (FPs) has been used as a bioorganic tool for the control of sheath blight disease of rice. Combined application of antagonistic microorganism showed that significant bio control activity and enhances plant growth by induced systemic resistance (ISR). The present study, we carryout morphological, physiological and biochemical analysis and then identified, the selected isolate VSMKU2 is Pseudomonas sp. Maximum level of phenylalanine ammonia lyase (PAL) was quantified in the treatment of Pseudomonas sp VSMKU2 + R. solani on the 7th day (97.50 nmol trans-cinnamic acid/min/g). Similarly, the cell free culture filtrate of VSMKU2 challenged with R. solani demonstrated lower level of PAL activity on 7th day (91.76 nmol trans-cinnamic acid/min/g) compared to control. Peroxidase (PO) and polyphenoloxidase (PPO) gave higher activity in Pseudomonas sp VSMKU2 challenged with R. solani on 7th day (0.94 and 0.95 unit/min/g of proteinrespectively) but 14th and 21st day after challenged inoculation of R. solani had been reduced (0.92, 0.75 and 0.82, 0.65 unit/min/g of protein) compared to control. The total phenol content activity was significantly increased with Pseudomonas sp VSMKU2 (148.27 µg catechol/mg/g of protein) and cell free culture filtrate of VSMKU2 (137 µg catechol/mg/g of protein) treated in rice seedlings on 7th day after challenged inoculation of R. solani compared to control. The results obtained in the current study imply to Pseudomonas sp VSMKU2 was able to rise defence response, thereby contribute resistance to sheath blight disease.