0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.12.3.70 Extraction of Chitin and Chitosan from Wild Type Pleurotus Spp and its Potential Application - Innovative Approach Johney Jesteena Radhai Sri S Ragunathan R Department of Food and Nutrition, Research and Development Centre, Bharathiar University, Coimbatore - 46, Tamilnadu, India. Department of Nutrition and Dietetics, PSG College of Arts and Science, Coimbatore -14, Tamilnadu, India. Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore - 21, Tamilnadu, India. 30 09 2018 12 3 1631 1640 Copyright © 2018 The Author(s) 2018 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.https://creativecommons.org/licenses/by/4.0/

Biopolymer of chitin and chitosan were extracted from the two Pleurotus spp. of P.floridanus and P.djamor. After the optimization, genomic DNA was isolated, and by using ITS 1 and ITS 4 primers molecular identification was carried out and the nucleotides were submitted to NCBI. The biopolymer have a great commercial importance now a days because of it high efficiency in all the field. The present study was focused on to extract the chitin and chitosan from the Pleurotus spp. and these polymer was subjected to analysis the total antioxidant activity, antimicrobial activity against food borne pathogens of E.coli, B. cereus, S.aureus, A.niger and A.flavus. Cytotoxicity study against HeLa Cell Line was also performed to study the efficacy of the extracted polymer.

Keywords Pleurotus spp chitin chitosan total antioxidant antimicrobial activity Cytotoxicity