0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.12.3.65 Optimization (Substrate and pH) and Anaerobic Fermentative Hydrogen Production by Various Industrial Wastes Isolates Utilizing Biscuit Industry Waste as Substrate Hari Dinesh Gujuluva Sundaram Karthik Mohanrasu Kulanthaisamy Murugan Ramu Satheesh Vinayaga Moorthi Puthamohan Angelin Swetha Tondi Rajan Selvakumar Gopal Arun Alagarsamy Department of Energy Science, Alagappa University, Karaikudi, India. Department of Microbiology, Alagappa University, Karaikudi, India. Department of Microbiology, PSG Arts College, Coimbatore, India. Department of Human Genetics and Molecular Biology, Bharathiyar University, Coimbatore, India. Department of Microbiology, DDE, Alagappa University, Karaikudi, India. 30 09 2018 12 3 1587 1595 Copyright © 2018 The Author(s) 2018 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.https://creativecommons.org/licenses/by/4.0/

The bio hydrogen (H2) production by anaerobic digestion of industrial waste is beneficial one due to the availability of proteins and carbohydrates as potential substrate for biological H2 production. An anaerobic fermentative route is a promising method of biohydrogenation. The microbial isolates from various industrial wastes (dairy, sugar and food) were assessed for their potential bio H2 production. The selected individual isolates (F1 - Bacillus subtilis and A3 -Bacillus subtilis) and their cocultures were used for the optimization of bio H2 production utilizing various concentration of biscuit industry waste as substrate at various pH conditions. The mixed consortium which displayed the higher bio H2 production was selected for the detailed analysis of the 3L fermentation studies using 90% Organic Loading Rate (OLR) substrate at pH 6.5. Significantly higher Hydrogen Yield (HY) of 0.87 mol H2/mol glucose on 16th day of incubation was observed.

Keywords Food waste substrate pH Bio hydrogen Fermentation Bacillus subtilis