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<article article-type="research-article" dtd-version="1.0" xml:lang="en"
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    <front>
        <journal-meta>
            <journal-id journal-id-type="issn">0973-7510</journal-id>
            <journal-title-group>
                <journal-title>Journal of Pure and Applied Microbiology</journal-title>
            </journal-title-group>
            <issn pub-type="epub">2581-690X</issn>
            <publisher>
                <publisher-name>DR. M.N. Khan</publisher-name>
            </publisher>
        </journal-meta>
        <article-meta>
            <article-id pub-id-type="doi">10.22207/JPAM.12.3.57</article-id>
            <title-group>
                <article-title>Role of Hydrolytic Enzymes of Some Fungi in Rotting Fruits and a Possibility of Retard Spoilage</article-title>
            </title-group>
            <contrib-group>
				<contrib contrib-type="author">
                    <name>
                        <surname>Moustafa</surname>
                        <given-names>S.M.N</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff-1"/>
					<xref ref-type="aff" rid="aff-2"/>
                </contrib>
                		
            </contrib-group>
			
			
            <aff id="aff-1">Biology Department, College of Science, Jouf University, P.O. Box: 2014, Sakaka, Saudi Arabia.</aff>
			<aff id="aff-2">Department of Botany and Microbiology, Faculty of Science, Minia University, El-Minia, Egypt.</aff>
	 
			
			
            <pub-date publication-format="electronic" date-type="pub" iso-8601-date="2018-09-30">
                <day>30</day>
                <month>09</month>
                <year>2018</year>
            </pub-date>
            <volume>12</volume>
            <issue>3</issue>
            <fpage>1525</fpage>
            <lpage>1535</lpage>
            <permissions>
                <copyright-statement>Copyright &#x00A9; 2018 The Author(s)</copyright-statement>
                <copyright-year>2018</copyright-year>
                <license license-type="open-access"
                    xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.<uri 
					xlink:href="https://creativecommons.org/licenses/by/4.0/"
                            >https://creativecommons.org/licenses/by/4.0/</uri></license-p>
                </license>
            </permissions>
            <self-uri xlink:href="https://www.microbiologyjournal.org/role-of-hydrolytic-enzymes-of-some-fungi-in-rotting-fruits-and-a-possibility-of-retard-spoilage/"/>
            <abstract>
                <p>This study was designed to identify some fungi responsible for damage of fruits in
storage and sale outlet markets. Results declared the role of CMC-ase and poly galacturonase
(PG) enzymes secreted by three fungi of Alternaria alternata, Penicillium italicum and
Rhizopus stolonifer in decomposition of tomato, lemon and cucumber fruits at the grocery
markets in Sakaka, Saudi Arabia. CMC-ase and PG were produced after 2 days for P. italicum,
R. stolonifer whereas very little quantity was detected for A. alternata. Maximum CMC-ase
and PG production were seen after 8-10 days of incubation at 28ºC. Enzyme production started
at a temperature of 5°C but in small quantities, then the production began to increase steadily
until the temperature of 28°C, which produced the highest amount of enzymes. The production
began to decline starting at 35ºC to be at the lowest rates at 40ºC. Enzymes were produced at
pH values of 4.5-9.5, with optima between 5.5-6.5 for the three tested fungi. Acidic medium of
pH 4.5, and alkaline medium (above pH 6.5) affected negatively on the production of enzymes,
and this effect was more evident in the exo CMC-ase and exo PG than in endo enzymes. Results
showed that the mixture of menthol crystals and Eucalyptus oil and camphor and methyl salicylate
oil-rich air inhibited growth of R. stolonifer and A. alternata by 100% and inhibited the growth
of P. italicum by approximately 50%, compared to the control sample. Camphor and mint
oils gave negative effect. Application of devices to emit the odor and quench this mixture of
menthol crystals and Eucalyptus oil and camphor and methyl salicylate to fumigate the air of stores
and outlets for selling vegetables and fruits are recommended.
		</p>
		</abstract>
		<kwd-group>
        <title>Keywords</title>
        <kwd>Alternaria alternata</kwd>
        <kwd>Enzymes, Essential oil</kwd>
			<kwd>Penicillium italicum</kwd>
			<kwd>Rhizopus stolonifer</kwd>
			<kwd>Rotted Fruits</kwd>
         </kwd-group>
        </article-meta>
    </front>
    </article>
