Chamomile (Matricaria chamomilla L.) is one of the nine important medicinal plants found in the world. In this study, the effect of different concentrations of plant growth regulators and explants has been investigated on embryogenesis and rooting of German chamomile (Matricaria spp.). MS culture medium (Murashige Skoog., 1986) was the basal medium in this experiment. Factorial experiments were conducted in a Randomized Complete Block Design with three replications. In testing the effect of 1-Naphthaleneacetic acid (NAA) and Kinetin on the embryogenesis, factors included: (NAA) at four levels (0, 0.5, 1, 2 mg/l), Kinetin at four levels (0, 0.5, 1, 2 mg/l) and explants in three levels: Stem, Axillary buds and leaves. In investigating the effect of NAA and Kinetin on rooting, factors included NAA at four levels: (0, 0.5, 1, 2 mg/l), Kinetin at four levels: (0, 0.5, 1, 2 mg/l). Results showed that leaf explants in culture medium containing a treatment Al: (NAA: 1 +Kin: 1mg/l) and stem explants in culture medium containing a treatment A2: (NAA: 1 +Kin: 0.5 mg/l) and the Axillary bud explants in culture medium containing hormonal composition of A3: (NAA: 0.5 +Kin: 1mg/l) produced the highest embryogenic calli. In terms of rooting, the culture medium containing the treatment A4: (NAA: 1 +Kin: 0 mg/l) created the highest rooting.