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<article article-type="research-article" dtd-version="1.0" xml:lang="en"
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    <front>
        <journal-meta>
            <journal-id journal-id-type="issn">0973-7510</journal-id>
            <journal-title-group>
                <journal-title>Journal of Pure and Applied Microbiology</journal-title>
            </journal-title-group>
            <issn pub-type="epub">2581-690X</issn>
            <publisher>
                <publisher-name>DR. M.N. Khan</publisher-name>
            </publisher>
        </journal-meta>
        <article-meta>
            <article-id pub-id-type="doi">10.22207/JPAM.12.3.23</article-id>
            <title-group>
                <article-title>Effect of Bioactive Compounds Extracted from Green Algae Spirogyra sp. in Reducing the Protoscolices
Viability In vitro Compared with Albendazole Drug</article-title>
            </title-group>
            <contrib-group>
				<contrib contrib-type="author">
                    <name>
                        <surname>M. Alkaabi</surname>
                        <given-names>Haider</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff-1"/>
                </contrib>
				
				<contrib contrib-type="author">
                    <name>
                        <surname>M. Almayali</surname>
                        <given-names>Hadi</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff-1"/>
                </contrib>

                		
            </contrib-group>
			
			
            <aff id="aff-1">University of AL-Qadisiyah, Faculty of Education, Biology Determent, Al-Dewania, Iraq.</aff>
	 
			
			
            <pub-date publication-format="electronic" date-type="pub" iso-8601-date="2018-09-30">
                <day>30</day>
                <month>09</month>
                <year>2018</year>
            </pub-date>
            <volume>12</volume>
            <issue>3</issue>
            <fpage>1221</fpage>
            <lpage>1231</lpage>
            <permissions>
                <copyright-statement>Copyright &#x00A9; 2018 The Author(s)</copyright-statement>
                <copyright-year>2018</copyright-year>
                <license license-type="open-access"
                    xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.<uri 
					xlink:href="https://creativecommons.org/licenses/by/4.0/"
                            >https://creativecommons.org/licenses/by/4.0/</uri></license-p>
                </license>
            </permissions>
            <self-uri xlink:href="https://www.microbiologyjournal.org/effect-of-bioactive-compounds-extracted-from-green-algae-spirogyra-sp-in-reducing-the-protoscolices-viability-in-vitro-compared-with-albendazole-drug/"/>
            <abstract>
                <p>The crude alcoholic extract of Spirugera sp. algae with concentrations (250, 500,
1000) mg/ml and albendazole with concentrations (250, 500, 1000) mg/ml effects was studied
in the viability of protoscolices in vitro, the viability of the protoscolices in 10 microliters and
the three replicates calculated in the fixed-size method. There were no significant differences
in the first three treatments (0,24,48 hour), whereas the coefficients of 72-144 hour showed
significant differences . The mean S.D. ± viability of the protoscolices at the highest rate at
treatment at 0 hour (33 ± 5.29) protoscolices, while the mean of the protoscolices dropped to
the lowest ratio at 144 to 14 ± 1 protoscolices. While the mean S.D.± mean of the protoscolices
viability of the treatments 24-120 hours between 31.33 ± 1.52-16.66 ± 1.52 protoscolices. The
total number of protosclices ranged from 177-193. The average number of live protoscolices
ranged between 170-177 protoscolices / 100 μl , While the number of dead protoscolices ranged
from 6-16 protoscolices / 100μl. Sixty-nine chemical compounds were extracted from the crude
alcoholic extract of Spirogyra sp. Varied between phenols, turbines, fatty acids, hydrocarbons
and hetero cyclic compounds. The results of this study showed a decrease in the percentage
of the viability of the protoscolices when exposed to the concentrations of the crude alcoholic
extract of the Spirugera sp. The concentration of 250 mg / ml resulted in a decrease in the
percentage of protoscolices from 92.75±0.62 at 0 hour to 27.06±0.48 at 144 hour compared
with control group (76.56 ± 0.79), while the concentration of 500 mg/ml decreased from
92.59±0.58 per hour to 0.14±0.25% at 144 hours compared with the control group, while the
concentration was 1000 mg /ml has led to the killing of all protoscolices at 144 hour compared
with the control group, The results of used albendazole drug showed significant differences.
concentrations reduced the percentage of viability of protoscolices. When exposed to 250 mg
/ ml, The result was a decrease from 93.75 ± 0.77 at 0 hour to 4.15 ± 0.36 at 144 hour, while
the concentration of 500 mg / ml reduced the percentage from 48 ± 0.85 at 0 hour to 0,4±0.48
at 144 hour, while The concentration of 1000 mg / ml resulted in the killing of all protpscolices
indications at 120 hour compared to 0 hour where the percentage of viability of protoscolices
was 45.5 ± 0.98. The results of this study showed that a similar effect of a Spirogyra sp extract
and albendazole drug on the viability of the protoscolices with increased concentration and
duration of exposure to the drug or alcoholic extract.
		</p>
		</abstract>
		<kwd-group>
        <title>Keywords</title>
        <kwd>Green Algae</kwd>
        <kwd>Spirugera</kwd>
			<kwd>Hydatid cysts Disease</kwd>
			</kwd-group>
        </article-meta>
    </front>
    </article>
