The present study envisaged to optimise the culture parameters for improved bioactive secondary metabolite production by Geosmithia pallida (KU693285), a potent endophytic fungi, isolated from endangered medicinal plant Brucea mollis Wall ex. Kurz. G. pallida was isolated from healthy, surface sterilized leaf, bark and root tissues of B. mollis. Sporulation was induced in sterile G. pallida by growing it on modified PDA media. Identification of G. Pallida was confirmed by ITS r-DNA sequence analysis. The culture parameters were optimized for enhanced production of metabolites. Maximum metabolite production was observed at 25°C incubation temperature and 6.5 pH on 28th day of inoculation, when lactose and yeast extract were added in the basal medium respectively as carbon and nitrogen source along with 3.5 g/l NaCl. The inhibition zone showed by G. pallida was significantly higher during the optimum culture conditions than that of the control (basal media). From the present study, it is concluded that for the maximum production of bioactive metabolite the culture parameters need to be optimized. These results would steer the authors to carry out further studies on purification, characterization, and identification of bioactive metabolites produced by G. pallida (KU693285) for pharmaceutical as well as agricultural applications.