0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.12.2.22 Efficacy of Different ELISA, Histopathology and PCR Assays for the Diagnosis of Ovine Brucellosis in Ram A. Hussein Muayad A. Muhammid Hayder G. AL-Shemmari Ihab J. Jameel Yasser Department of Pathology, College of Vet.Med. University of Kerbala. Iraq. Department of Microbiology, College of Vet.Med. University of Kerbala. Iraq. Department of Vet.Internal and Preventive medicine, College of Vet.Med, University of Kerbala. Iraq. Department of Public, College of Vet. Med., University of Kerbala, Iraq. 30 06 2018 12 2 641 646 Copyright © 2018 The Author(s) 2018 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.https://creativecommons.org/licenses/by/4.0/

Diagnosis of ovine brucellosis infection inthe ram hasepididymitis and orchitis has not been established. The aim of this study,to investigate the diagnostic value of ELISA, Histopathology and PCR for ovine brucellosis in ram in Karbala city. 30 ram’s serum and semen were collected from ram for serological and molecular assay, in addition three testicular tissueswere castrated from ram forhistopathological diagnosis of Ovine brucellosis. Sensitivity and specificity of ELISA and PCR were 80% ,70%; and 87.5%, 57.14%; respectivel,Palpable enlargement of the epididymis and testicular hypoplasia. Sensitivity by ELISA was higher in the ovine brucellosis detection than PCR, but specificity values were lower. Grossly visible lesions in the epididymis associated with Ram Brucellosis withPocket of inspissate material resulting from extravasation of sperm.

Keywords ELISA histopathology PCR molecular tests brucellosis Ram