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<article article-type="research-article" dtd-version="1.0" xml:lang="en"
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    <front>
        <journal-meta>
            <journal-id journal-id-type="issn">0973-7510</journal-id>
            <journal-title-group>
                <journal-title>Journal of Pure and Applied Microbiology</journal-title>
            </journal-title-group>
            <issn pub-type="epub">2581-690X</issn>
            <publisher>
                <publisher-name>DR. M.N. Khan</publisher-name>
            </publisher>
        </journal-meta>
        <article-meta>
            <article-id pub-id-type="doi">10.22207/JPAM.12.2.14</article-id>
            <title-group>
                <article-title>Milk Decontamination and an Effective Milk Pasteurizing Method for Household Dairies</article-title>
            </title-group>
            <contrib-group>
				<contrib contrib-type="author">
                    <name>
                        <surname>O. Olajuyigbe</surname>
                        <given-names>Olufunmiso</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff-1"/>
					<xref ref-type="aff" rid="aff-2"/>
                </contrib>
				
				<contrib contrib-type="author">
                    <name>
                        <surname>O. Adeoye-Isijola</surname>
                        <given-names>Morenike</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff-1"/>
                </contrib>
				
				<contrib contrib-type="author">
                    <name>
                        <surname>C. Iyashere</surname>
                        <given-names>Tochukwu</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff-1"/>
                </contrib>
				
				<contrib contrib-type="author">
                    <name>
                        <surname>Adedayo</surname>
                        <given-names>Otunola</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff-1"/>
                </contrib>
				
				<contrib contrib-type="author">
                    <name>
                        <surname>J. Afolayan</surname>
                        <given-names>Anthony</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff-2"/>
                </contrib>
				
				<contrib contrib-type="author">
                    <name>
                        <surname>M. Coopoosamy</surname>
                        <given-names>Roger</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff-3"/>
                </contrib>
                		
            </contrib-group>
			
			
            <aff id="aff-1">Department of Microbiology, School of Sciences and Technology, Babcock University, PMB 4005, Ilishan Remo, Ogun State, Nigeria.</aff>
			<aff id="aff-2">Medicinal Plants and Economic Development Research Centre, University of Fort Hare, Alice, 5700, Eastern Cape, South Africa.</aff>
			<aff id="aff-3">Department of Nature Conservation, Faculty of Natural Sciences, Mangosuthu University of Technology, Umlazi, Durban, South Africa.</aff>
	 
			
			
            <pub-date publication-format="electronic" date-type="pub" iso-8601-date="2018-06-30">
                <day>30</day>
                <month>06</month>
                <year>2018</year>
            </pub-date>
            <volume>12</volume>
            <issue>2</issue>
            <fpage>555</fpage>
            <lpage>566</lpage>
            <permissions>
                <copyright-statement>Copyright &#x00A9; 2018 The Author(s)</copyright-statement>
                <copyright-year>2018</copyright-year>
                <license license-type="open-access"
                    xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.<uri 
					xlink:href="https://creativecommons.org/licenses/by/4.0/"
                            >https://creativecommons.org/licenses/by/4.0/</uri></license-p>
                </license>
            </permissions>
            <self-uri xlink:href="https://microbiologyjournal.org/milk-decontamination-and-an-effective-milk-pasteurizing-method-for-household-dairies-2/"/>
            <abstract>
                <p>This study investigated the bacteriological and the hygienic properties of some
commonly sold canned milk and determined the effectiveness of pasteurization of milk
contaminated with bacterial isolates spectrophotometrically. In this study, Escherichia coli,
Streptococcus spp., Enterococcus aerogenes, Proteus spp., Clostridium spp., Lactobacillus spp.
and Staphylococcus aureus were isolated from the milk samples. The bacterial load of the milk
samples ranged from 5 x 105 to 22 x 105 cfu/ml. The methylene reduction time indicating the
hygienic quality of each milk sample varied from one milk to the other. While NNM and VSM
samples not decolourized after 8 h were considered excellent and PNM and OFM decolourized
methylene blue after 6 and 7 h respectively were considered good milk samples, CFM and
TCM that decolourized methylene blue after 4 and 5 h respectively were considered fair milk
samples. None of them was considered poor as they were not decolourized in less than 2 h.
Pasteurizing milk contaminated with indigenous microflora (IMF), E. coli and Strep. pyogenes
at 40oC, 50oC, 60oC and 70oC over a period of 30 min showed a varied degree of reduction in
the absorbances and its effectiveness. A 10oC increase in temperature resulted in significant
reduction in the absorbances obtained at different time intervals for all the milk samples. This
investigation shows that pasteurizing milk with low heating would reduce the microbial loads
of the milk samples. The method of pasteurizing milk in this study would be applicable in the
household dairies where sophisticated equipment are not required to control microbial loads
in milk.
		</p>
		</abstract>
		<kwd-group>
        <title>Keywords</title>
        <kwd>Contamination</kwd>
        <kwd>methylene blue reduction</kwd>
			<kwd>indigenous microflora</kwd>
			<kwd>pasteurization</kwd>
         </kwd-group>
        </article-meta>
    </front>
    </article>
