0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.11.4.32 Culture Based Isolation of Pathogenic Bacteria Associated with Respiratory Disease Complex in Broiler with Special Reference to Ornithobacterium rhinotracheale from India Patel J.G Patel B.J Joshi D.V Patel S.S Raval S.H Parmar R.S Chauhan H.C Chandel B.S Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat, India. Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat, India. Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat, India. 30 12 2017 11 4 1919 1924 Copyright © 2017 The Author(s) 2017 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.https://creativecommons.org/licenses/by/4.0/

In this study, isolation and identification of pathogenic bacteria, with special reference to Ornithobacterium rhinotracheale associated with respiratory diseases complex (RDC), were performed from a total of 60 biomaterials collected from healthy and diseased broilers of commercially reared farms in and around Palanpur, Banaskantha, Gujarat. Prevalence of RDC was 6.67% and 50.00% in apparently healthy and diseased broilers respectively. The incidence of E.coli, Staphylococcus spp., Ornithobacterium rhinotracheale, Pasteurella spp. and Klebsiella spp. were 8 (47.06%), 4 (23.53%), 3 (17.65%), 1 (5.88%) and 1 (5.88%) in broilers respectively. Highest bacteria were isolated from lung (58.33) followed by trachea (41.66). Ornithobacterium rhinotracheale, one of the causative agents of the emerging respiratory diseases complex of broiler could be isolated either singly or concurrently with other bacteria such as Escherichia coli, Klebsiella spp. and Pasteurella spp. indicating its possible etiological role in respiratory disease.

Keywords Respiratory diseases complex isolation Ornithobacterium rhinotracheale broilers