0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.11.4.29 Study of Erythromycin Resistance Genes in Staphylococcus aureus Isolated from Throat Swab Culture Mohamed Abulkasim Halemah Shukla G.S Bajaj H.K Masih Harrison Department of Clinical laboratory Sciences-Faculty of Health Science, SHUATS, Allahabad-211007. U.P., India. 30 12 2017 11 4 1891 1897 Copyright © 2017 The Author(s) 2017 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.https://creativecommons.org/licenses/by/4.0/

Throat swabs were collected to isolate S. aureus by using blood agar media. Biochemical tests were performed. Genomic DNA was isolated and PCR was done for erm A, ermB and erm C genes followed by bioinformatics analysis. Isolates were screened on erythromycin disc (erythromycin range 1- 125mg/l). All 47 S. aureus isolates found to be resistance to erythromycin by phenotypic methods contained at least 1 were erythromycin resistance gene. That’s mean all S. aureus isolates which were resistance to erythromycin by disc diffusion method were found as positive by PCR for erm A, B, and C genes.

Keywords Throat swabs bacteria ermA ermB ermC