0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.11.4.17 Gold Nanoparticles and Laser as Antimicrobial Agents against Some Types of Bacteria S.M. Al-Shukri Maysa Department of Microbiology, College of Medicine, Babylon University, Hilla, Iraq. 30 12 2017 11 4 1783 1788 Copyright © 2017 The Author(s) 2017 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.https://creativecommons.org/licenses/by/4.0/

The goal of this study was to assess the efficacy of gold nanoparticles (AuNPs) and AuNPs– Low level laser combined therapy as antibacterial agents against different types of bacterial isolates in vitro. Five concentrations of AuNPs (25,50,75, 100 and 200 μg/mL) were employed for assessing bacterial growth rate and the bacterial MIC. It was found that there was slight reduce in bacterial growth after treatment with gold nanopracticle. The influence of gold nanoparticles on Staphylococcus aureus biofilms was also studied. It was found that biofilm growth was decreased at different concentrations of AuNPs compared to absence of nanoparticles. Results of the cytotoxic act of AuNPs and low level laser effect in combination against Escherichia coli and S. aureus referred that the low level laser light improved the antibacterial action of AuNPs when it used in combination with low level laser.

Keywords Gold nanoparticles Laser Antibacterial agent Cytotoxicity MIC