0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.11.3.20 Immunization Intraductal Fibronectin and Whole Cells of Staphylococcus aureus, Escherichia coli and Streptococcus agalactiae, in Cows to Dry in Puebla-Mexico Perez Martel S.L Romero O Trevino I Delgado G Petlacalco B Romero F Hernandez H De Ita Ma. A Valencia Rodriguez-Hernandez V Colegio de Posgraduados, Campus Puebla-Mexico. Centro de Agroecologia-ICUAP; Benemerita Universidad Autonoma de Puebla-Mexico. Facultad de Medicina Veterinaria-BUAP. CyTA Labs. 30 09 2017 11 3 1387 1392 Copyright © 2017 The Author(s) 2017 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.https://creativecommons.org/licenses/by/4.0/

Nine dairy cows seven months gestation received via the milk ducts formulation inactivated antigen protein adhesion to fibronectin of Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli cells. The analysis of the immunoglobulins G by indirect ELISA against the applied antigens, showed that five of nine cows were stimulated with Escherichia coli, the case for the antigenic stimulus for Streptococcus agalactiae showed that eight of nine cows responded with good indices of significance. Finally, stimulus virulence proteins of Staphylococcus aureus adhesion to fibronectin, revealed response rates all cows (P < 0.0002). Intraductal response to antigens promises to be a way for immunization to dairy cows in the drying stage.

Keywords Acute mastitis inactivated antigens analysis of immunoglobulins