0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.11.3.06 Removal of Erionyl blue A-R and Solophenyl Black FR Textile Dyes using Enzymatic Extracts of Laccases of Pleurotus ostreatus and Pleurotus djamor Alonso-Calderon AIA Chavez-Bravo E Vidal-Robles E Martinez-Galindo S Omaire-Vargas J.R Marin-Castro M.A Chemical Engineering Faculty, Autonomous University of Puebla, Mexico. University City, Avenue San Claudio and south 18, Colony San Manuel. Center for Research in Microbiological Sciences,Institute of Sciences Autonomous University of Puebla, Mexico. Chemical Engineering Faculty, Autonomous University of Puebla, Mexico. Engineering Faculty, Autonomous University of Puebla, Mexico. Agricultural Sciences Research Department of the Sciences-Institute,Autonomous University of Puebla, Mexico. 30 09 2017 11 3 1275 1279 Copyright © 2017 The Author(s) 2017 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.https://creativecommons.org/licenses/by/4.0/

In this research, extracellular fungal extracts containing Pleurotus ostreatus and Pleurotus djamor were used for the removal of erionyl blue A-R (anthraquinone) and solophenyl black FR (azo) textile dyes in artificially contaminated water at 200 ppm. For erionyl blue A-R, removals of up to 93.6 and 42.85% were achieved, and for solophenyl black FR of 27 and 31.14%, using the enzymatic extracts of P. ostreatus and P. djamor respectively. Enzymatic activity values of 888.41 IU and 152.22 IU were reached for the laccases obtained from the submerged culture extract of Pleurotus ostreatus and Pleurotus djamor respectively. The extract obtained from P. ostreatus was partially purified using dialysis and anion exchange chromatography (DEAE), by polyacrylamide gel electrophoresis. A molecular mass of 67 kDa was determined.

Keywords Pleurotus dye laccase enzyme