This study was aimed to know the prevalence of biotypes, serotypes and phage types and the antibiotic susceptibility pattern of Vibrio cholerae isolates in our region.1,975 consecutive diarrheal stool samples were processed between 2009 and 2013. Standard microbiological methods and guidelines were followed to isolate, identify Vibrio cholerae and to perform antibiotic susceptibility test. Serotypes, biotypes and phage types of the isolates were determined. 106 Vibrio cholerae O1 strains were isolated from 1,975 stool samples (5.36%). All isolates were El Tor biotype. Predominant serotype was Ogawa (101/106, 95.2%). Phage types T2 (Basu and Mukerjee) and T27 (new scheme) were most common. High level of resistance was seen towards ampicillin and Co-Trimoxazole. Least resistance was towards chloramphenicol. No specific trend was shown against tetracycline. To conclude, V. cholerae O1 El Tor, biotype Ogawa was the most common serotype found in this area. Doxycycline still remains the drug of choice. Antibiotic susceptibility pattern of V. cholerae must be monitored by tertiary care centers.