Most isolates of the genus Trichoderma were found to act as mycoparasites of many economically important aerial and soil-borne plant pathogens. Trichoderma has gained importance as a substitute for chemical pesticides and hence an attempt was intended to corroborate the positive relatedness of molecular and morphological characters. A fungal strain of Trichoderma asperellum CA-03/9840 was isolated from a soil sample collected from Farmer Field, Sultanpur ,Uttar Pradesh, India. The universal primers were used for amplification of 5.8SrRNA gene fragment and the strain was characterized by using 5.8SrRNA gene sequence with the help of ITS marker. It is proposed that the identified strain Trichoderma asperellum CA-03/9840 be assigned as the type strain of a species of the genus Trichoderma based on Tricho Key analysis together with the 5.8SrRNA gene sequence search in Ribosomal Database Project, small subunit rRNA and large subunit rRNA databases. The sequence was deposited in Gene Bank with the accession number KU821782. Thus an integrated approach of morphological and molecular markers can be employed to identify a superior strain of Trichoderma for its commercial exploitation.