0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.10.4.13 Screening of Antenatal Lower Genital Tract Infection in Women of Saudi Arabia for Preventing Preterm Birth and Low Birth Weight using PCR Techniques Al- Hajjaj Yasser Abdullah Al-ssum Rashead Musa Shair Omar H. M. Askari Ejaz Hatamleh Ashraf Atef King Saud University, College of Science, department of Botany and Microbiology, Riyadh, Saudi Arabia. King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia. King Saud University, College of Science, department of Botany and Microbiology, Riyadh, Saudi Arabia. 30 12 2016 10 4 2577 2583 Copyright © 2016 The Author(s) 2016 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.https://creativecommons.org/licenses/by/4.0/

The aim of this study was to detect bacterial infection of: Chlamydia trachomatis, Ureaplasma spp. and Mycoplasma, from Saudi women samples. Samples were 100 from pregnant women attending the obstetrics and Gynecology clinic at Alymamah Hospital, Riyadh, Saudi Arabia during March 2013 to March 2014. DNA was successfully amplified from 56 samples. The results of this study indicated more reliable method which detected Chlamydia trachomatis, Ureaplasma spp. and Mycoplasma which caused vaginitis in lower genital, showed high frequency of both Mycoplasma spp (44.6%) and Ureaplasma spp (30.4%) infection among Saudi pregnant women. Association of Mycoplasma spp, Ureaplasma spp, and C. trachomatis has been observed but no association was observed between M. hominis, M. genitalium and U.urealyticum.

Keywords Chlamydia Mycoplasma Ureaplasma Vaginitis