0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.10.4.10 Multilocus Sequence and spa Typing for Characterization of Staphylococcus aureus Methicillin-Sensitive Isolated From Bovine Mastitis Cases in Algeria Dairy Farms Benhamed Nadjia Philippe Gautier Ismahene Mezouar Yves Donnio Pièrre Mebrouk Kihal Department of Applied Molecular Genetics, Faculty of Nature and Life Sciences, University of Sciences Technologies of Oran –Mohammed Boudiaf (USTO-MB), BP 1505 El M’naouar Bir Eldjir Oran 31000, Oran, Algeria. Laboratory of Applied Microbiology, Department of Biology, Faculty of Nature and Life Sciences, University Oran 1, Ahmed Ben Bella, BP 16, ES-senia 31000,Oran, Algeria. Unit of Microbiology, University of Rennes 1, 35043, Rennes, France. 30 12 2016 10 4 2557 2562 Copyright © 2016 The Author(s) 2016 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.https://creativecommons.org/licenses/by/4.0/

Staphylococcus aureus causes one of the most common types of chronic mastitis. Mastitis is an inflammation of the mammary gland with local and symptoms that occasionally result in a systemic infection. This disease has profound impact on animal welfare and milk quality. This study allowed us to determine the MLST and spa typing diversity of Staphylococcus aureus isolated from cases of bovine mastitis in the west Algeria. The first purpose of this work was to investigate the phylogenetic profile of Staphylococcus aureus by spa typing and Multilocus sequence typing (MLST) isolated from Mastitis subcliniques and clinical cases which were previously investigated by Mass Spectrometry Maldi-Tof and spa typing. The second purpose was to determinate the complex clonal of strains. In total, 213 quarters milk samples were collected from 56 dairy cows at 6 farms in the region of Oran, from 2011 to 2012. The quarter milk samples were bacteriological analyzed and scored using California Mastitis Test. The prevalenceisolated bacteria were Staphylococci (38.9%).

Keywords Mastitis Staphylococcus aureus identification MLST spa phylogeny