The predominance of bacteria on door handles and computers keyboards in Faculty of Science, University of Kufa in Najaf Governorate was assessed. One hundred samples were collected and cultured for bacterial identification. The occurrence of positive samples was as the following; 95% with both of toilets doors handles and computers keyboards, 90% in laboratories doors handles, 80% in Classrooms doors handles and 75% in offices doors handles. The current study demonstrate a high prevalence rate of aerobic bacteria on different doors handles and computers keyboards in Faculty of Science, University of Kufa. The current study gave a clear view about the microbial contamination of door handles and computer keyboards and the possibility to be one of the main sources of infection in the university environment.