0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.13.1.26 Determination of Symbiotic Effectiveness of Rhizobium Strains Isolated from Food Legumes (Bean) Collected from Fez, Morocco Berrada Halima Ibijbijen Jamal Fikri-Benbrahim Kawtar Laboratory of Microbial Biotechnology, Sciences and Technology Faculty, Sidi Mohammed Ben Abdellah University, P.O. Box 2202, Fez, Morocco. Environment and Soil Microbiology Unit, Faculty of Sciences, Moulay Ismail University, P.O. Box 11201 Zitoune, Meknes, Morocco. 09 03 2019 13 1 247 255 Copyright © 2019 The Author(s) 2019 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. https://creativecommons.org/licenses/by/4.0/

This study was conducted in order to evaluate the symbiotic effectiveness of Rhizobium strains isolated from food legumes (Bean) collected from different sites in Fez city and regions (Morocco). For this purpose, 15 isolates were obtained. Seeds were inoculated with these isolates and grown in bags containing sterile soil in greenhouse under controlled plant growth conditions. 30 days after planting several measurements were carried out to assess nodulation, plant growth and N2 fixation. Nodule number and dry weight were determined as an indication of inoculation response; shoot and Root dry weights as an indication of growth response, and N-uptake as an indication of nitrogen fixation effectiveness. The results of the experiment were statistically analyzed showed a statistically significant difference between the tested strains (P < 0.05).

Keywords Bean Rhizobium N2 Fixation efficiency