The growing threat of Multi-resistant (MDR) P. aeruginosa had been widely distributed through infection of chronic wounds, urinary tract, and diabetic feet and also nosocomial infection of hospitals. This study was planned to solve the problem of the resistance of MDR P. aeruginosa to most recent antibiotic in pseudomonal infected patients, and to produce a new treatment of natural source which has anti bacterial activity and wound healing effect with no side effect as chitosan is a natural polymer used widely in wound management with its unique non toxic nature. Cinnamon oil has a variety of medical a applications as anti-microbial, anti -ulcers and anti- inflammatory effect. Our study included 100 patient admitted to Alexandria University Hospital with different wound and ulcers infections. Total of 127 pathogens were isolated, 43 (30%) were P. aeruginosa. Most resistant six P. aeruginosa isolates were chosen for separated study. Chitosan and cinnamon oil were prepared from their natural sources. The results ended with a final formula of some natural component which has anti-bacterial activity, non-inflammatory, wound healing activity, with no toxic side effects. It could be included that the recent intensive used antibiotics can be replaced with the prepared natural formula as it can help in the full recovery of the wounds faster and safer than antibiotics.