ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
Muslim Suardi1,2, Annisa Suri Hamdani1, Bertiliyus Ariyati1, Rika Sari Lalfari3, Dede Lutfrian1, Asiska Permata Dewi4, Rizka Permata Suci5 and Akmal Djamaan1
1Faculty of Pharmacy, University of Andalas, Padang, Indonesia.
2Department of Pharmacy, University Mohammad Natsir, Bukittinggi, Indonesia.
3Department of Chemistry, Faculty of Sciences, University of Andalas, Padang, Indonesia.
4Department of Pharmacy, Faculty of Medicine and Public Health, Abdurrab University, Pekanbaru, Indonesia.
5Department of Chemistry, Faculty of Sciences, University of Indonesia, Jakarta, Indonesia.
J Pure Appl Microbiol. 2018;12(3):1163-1169
https://doi.org/10.22207/JPAM.12.3.15 | © The Author(s). 2018
Received: 17/06/2018 | Accepted: 13/08/2018 | Published: 30/09/2018
Abstract

Utilization of rice straw (Oryza sativa Linn) as a raw material in the production of poly(3-hydroxybutyrate), P(3HB), biopolymer by fermentation using Pseudomonas aeruginosa has been conducted. Rice straw stew and microcrystalline cellulose obtained from rice straw were used as carbon source for bacterial growth at various concentrations. The content of P(3HB) in the biomass was determined by gas chromatography using the RTX-1 column and the FID detector. Results showed that the best condition in producing P(3HB) was using 1% microcrystalline cellulose as carbon source and duration of fermentation of 48 hours. The biomass and P(3HB) obtained from MCC were  982 mg/100 mL and  17.19% w/w, respectively. While using rice straw stew were 269 mg/100 mL and 1.73%, respectively.  It can be concluded that the highest P(3HB) produced was found using microcrystalline cellulose obtained from rice straw waste as a carbon source.

Keywords

Rice straw, poly(3-hydroxybutyrate), Pseudomonas aeruginosa, Microcrystalline cellulose

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