ISSN: 0973-7510

E-ISSN: 2581-690X

M. Kumar, V.C. Suvarna and D. Radhakrishna
Department of Agril. Microbiology. UAS, GKVK, Bangalore – 560 065, India.
J Pure Appl Microbiol. 2011;5(1):421-424
© The Author(s). 2011
Received: 07/10/2010 | Accepted: 19/11/2010 | Published: 30/04/2011
Abstract

With an increasing demand for energy, alternate sources other than the conventional resources need to be explored. Bio-ethanol seems to be a promising substitute. Hence, an attempt was made to use jackfruit (Atrocarpus heterophyllus. Lam) seeds as a substrate for ethanol production. Amylolytic bacteria were isolated from different sources like carrot, tomato, potato, farm yard manure, forest litter and vermicompost. These isolates were used to saccharify the complex polymers to release fermentable sugars. Further, yeasts were isolated from grapes and distillery effluents and isolates were used to ferment sugars to yield ethanol.

Amylolytic bacterial isolate from Forest litter (FL1) yielded the highest quantity of reducing sugars (13.4 g /100g). Yeast isolate from grapes (YG1) yielded the highest quantity of ethanol (4.3 g /100 g) followed by distillery effluent isolate (YD1) yielded 3.8 g / 100g. Synergistic effect was observed with dual inoculation of FL1and YG6 resulting in ethanol production of 6.4 g /100g. Hence, dual inoculation with amylolytic and fermentative culture is best option to produce bioethanol.

Keywords

Saccharification, Fermentation, Residual sugars, Reducing sugars

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© The Author(s) 2011. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.