Two Step Production of Optimized Interferon Beta 1b; A Way to Overcome its Toxicity

ISSN: 0973-7510

E-ISSN: 2581-690X

Mohammad Ali Mobasher^1,2, Younes Ghasemi^1,2 , Nima Montazeri-Najafabady^1,2, Abdollah Ghasemian^1,2, Sara Rasoul-Amini^1,2,3, Shiva Hemmati^1,2 and Sirus Ebrahimi⁴

¹Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 71345 1583, Shiraz, Fars, Iran.

²Department of Pharmaceutical Sciences Research Center, School of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 71345 1583, Shiraz, Fars, Iran.

³Department of Medicinal Chemistry, Faculty of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 71345 1583, Shiraz, Fars, Iran.

⁴Department of Chemical Engineering, Sahand University of Technology, P.O. Box 53317 11111, Tabriz, Iran.

J Pure Appl Microbiol. 2013;7(4):2867-2871

Received: 21/01/2013 | Accepted: 25/02/2013 | Published: 30/12/2013

Abstract

Interferons are a large group of biological substances with many important functions like immunomodulatory, antiviral and anti inflammatory activities. There are so many clinical applications for IFN b for example in treatment of multiple sclerosis (MS), hepatitis, genital condylomata acuminate and arthritis. In this study the optimized IFN b 1b for expression in E.coli BL21 was designed and coloned in pET15b. In general, because of its toxicity and instability the yield of expressed IFN b 1b is low, so the amounts of expression and the effect of co products on production of IFN b 1b were evaluated and a two step production condition was designed for more efficacy in expression of this important biological recombinant product.

Keywords

Interferon, Optimization, cloning, Multiple sclerosis, E. coli

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