Farah Ayad, Amaria Matallah-Boutiba, Omar Rouane–Hacene,
Mohamed Bouderbala and Zitouni Boutiba
University of Oran 1 Ahmed Ben Bella, Laboratoire Réseau de Surveillance Environnementale (LRSE), Departement of Biology, BP 1524 El M’naouer, 31000, Oran, Alegria.
Western Algerian waste line is a dump of any form of pollution. Therefore sampling sites are ports, also close to urban waste which shelter a wide variety of fungal population. After seawater samplings, isolation we screened 235 kind of fungi for their behaviors with (Pb, Zn, Cd and Cu). Fourteen Trichoderma sp. isolates were evaluated for their tolerance to heavy metals stress at different concentrations of (Pb, Zn, Cd and Cu). Trichoderma sp. was exposed to concentrations between (0 – 4000 mg/l). The stress of biomass caused by the presence of heavy metals remains minimal. Maximum optimal growth has been demonstrated in our study at high concentrations of metals. Fungi expand gradually in cultures of metallic solutions. Its fresh weight reached 1, 89 Gr on 200 mg/l and 0,556Gr on 2000 mg/l. In the Zinc (Zn So4) solution the mycelium will grow mostly on 600 mg/l his weight achieved 0,97 Gr. Development in copper (Cu So4) solution is minimal, the fungi has pushed on 2000 mg/l to reach 0,046Gr. The Trichoderma sp. show an important potential of tolerance, it growth and stays different comparing to others fungus; The results emphasized the heavy metals detoxification abilities of Trichoderma sp. Bioremediation strategy could enhance the treatment of contaminated areas.
Keywords: Marine, Trichoderma sp., biomass, tolerance, heavy metals, western Algeria.