ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Saeed Arrak Turkey1, Ahmed Abdul-Hassan Abbas1* , Huda Dh. Hathal1 and Enas Adnan Abdulrasul2
1Department of Microbiology, College of Medicine, AL-Nahrain University, Baghdad, Iraq
2Department of Obstetrics & Gynecology College of Medicine, AL-Nahrain University, Baghdad, Iraq
J Pure Appl Microbiol, 2019, 13 (2): 1151-1157 | Article Number: 5644
Received: 15/04/2019 | Accepted: 25/05/2019 | Published: 30/06/2019
Abstract

The activity of TLRs as non-specific immunity in defense against T. gondii infections was observed particularly for TLR4 molecules. Several single-nucleotide polymorphisms (SNPs) residing in tow genes encoding these receptors were reported as significant genetic modifications of TLRs and associated with different pregnancy disorders. This study aimed to investigate the association between two single nucleotide polymorphisms (SNPs) in the toll-like receptor 4 gene (Asp299Gly and Thr399Ile) and susceptibility for toxoplasmosis. A total of 50 aborted women with IgM seropositive for Toxoplasma gondii and 50 aborted women with IgM seronegative for Toxoplasma gondii as controls were included in this study. DNA was extracted from the blood samples taken from these participants. TLR4 gene was amplified with polymerase chain reaction (PCR) using specific primers. Genotyping of the SNPs of interest were subjected for direct sequencing by (Macrogen /Korea), and the resultant sequences were compared with reference sequences in NCBI by using Bio edit software. The frequency of the heterozygous genotype (AG) was higher in patients than controls (20% versus 6%) with a significant difference (OR=3.92, 95%CI=1.01-15.22, p=0.037). Among the haplotype blocks, only ACC was significantly less frequent among patients than controls (85% versus 94%, p=0.038), while frequencies of other haplotypes were very close between patients and controls. The mutant allele (G) of the SNP Asp299Gly may be considered as a risk factor for toxoplasmosis and stimulate abortion in pregnant women.

Keywords

Toxoplasmosis, single nucleotide polymorphism, toll-like receptor 4.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.