Many species of filamentous fungi are known to survive in the Dead Sea, but the presence or absence of keratinophilic fungi in this hypersaline lake has not been investigated. In this work, 10 water samples were collected from different locations in the Dead Sea. All samples were studied for the detection of keratinophilic fungi using two procedures in which three different keratin sources and various incubation temperatures were employed. Dead Sea water samples inoculated with dermatophytes were included as positive controls. Results indicated the absence of these fungi from all Dead Sea water samples studied regardless of the keratin source used or the incubation temperature employed. Results indicated that fears of acquiring skin fungal infections “particularly dermatophytoses” as a result of swimming in the Dead Sea are not justified.