J Pure Appl Microbiol | Research Article | Volume 12, Issue 4 | Article Number: 5268

Manikandan Madheslu1, Ashok Kumar Marimuthu1, Vaishnavi Jagannathan1, Duraimurugan Kasiviswanathan2, Baby Shakila Parimelazhagan1 and Narendhran Sadasivam1*

1Department of Biotechnology, Sri Krishna Arts and Science College, Coimbatore – 641 008, Tamil Nadu, India.
2School of Community Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur – 711 103, India.

Corresponding Author E-mail: narendhransumathi@gmail.com
Received: 10/10/2018| Accepted: 21/11/2018 |Published: 31/10/2018
DOI: http://dx.doi.org/10.22207/JPAM.12.4.34

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ABSTRACT

Genus Streptomyces under phylum actinobacteria has been recognized as a prolific source for production of bioactive secondary metabolites. Actinobacterial strain designated as SKCMM1 isolated from Pichavaram Mangrove forest sediment was identified as Streptomyces cavourensis using polyphasic taxonomic approach. This strain shares 99% sequence similarity with Streptomyces cavourensis NBRC 13026T. Ethyl acetate fraction of the strain SKCMM1 exhibited highest biological activity. FTIR and GCMS analysis of crude compounds isolated from the active ethyl acetate fraction states the presence of several phenolic, hydrocarbon and fatty acid compounds with various bioactivities.  This study will be an attempt to understand that the strain holds significant antimicrobial activity against test pathogens and antiproliferative activity against HeLa cell lines (IC50 value of 8.9µg/ml).

Keywords: Streptomyces, Polyphasic Taxonomy, Liquid – liquid extraction, Spectral analysis, Bioactive compounds.