ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Sree Samanvitha K.1 , Sanjay Kumar S.1, Antony V. Samrot2 , Raji P.3, Ponnaiah Paulraj2, Iyappan P.2, Chandramohan M.2, Jenifer Selvarani A.3 and Durga Sruthi P.3
1Department of Biotechnology, Shanmugha Arts, Science, Technology & Research Academy, Thanjavur, India.
2Department of Biomedical Sciences, Faculty of Medicine and Biomedical Sciences, MAHSA University, JalanSP2, Bandar Saujana Putra, 42610, Jenjarom, Selangor, Malaysia.
3Department of Biotechnology, Sathyabama Institute of Science and Technology, Chennai – 600 119, India.
J Pure Appl Microbiol, 2019, 13 (3): 1841-1846 | Article Number: 5772
https://doi.org/10.22207/JPAM.13.3.61 | © The Author(s). 2019
Received: 01/07/2019 | Accepted: 22/08/2019 | Published: 22/09/2019
Abstract

AHL (acyl homoserine lactone) is a signaling molecule responsible for communication in gram negative bacteria, which is liable for bacterial virulence as well as biofilm formation. This study aims in targeting the AHL formation and AHL mediated virulence factor using plant extracts / plant metabolites. AHL plays a major role in pigment production, motility and rhamnolipid production in Pseudomonas aeruginosa. Inhibition of these mechanism by any mean implies that it is having impact in biofilm formation too. Plant derived metabolites/ extracts were subjected for swarming motility assay, the metabolite which inhibited the swarming motility was subjected for further study, where P. aeruginosa SU-3 was allowed to grow in the presence of the metabolite and its impact over the AHL production, pigment production and rhamnolipid production was evaluated. Curcumin was found to be effective against all the metabolites used. Thus curcumin subjected for molecular docking against ENR (enoyl carrier protein reductase), the enzyme involves in fatty acid synthesis and production of AHL.

Keywords

Pseudomonas aeruginosa SU-3; acyl homoserine lactone (AHL); rhamnolipid; pyocyanin pigment; enoyl reductase; molecular docking.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.