ISSN: 0973-7510
E-ISSN: 2581-690X
The succession of eukaryotic communities during traditional composting of domestic waste was analyzed through denaturing gradient gel electrophoresis (DGGE) combined with 18S rRNA gene fragment sequences. The 18S rRNA gene fragments were amplified through culture-independent and culture-dependent approaches. DGGE profiles showed that there were variations of microorganism community profiles during the initial of mesophilic, thermophilic, cooling and maturation phases. Further analysis based on 18S rRNA gene sequence showed that eukaryotic communities observed in the initial mesophilic phase were dominated by Ascomycota in addition of Stramenopiles and Uncultured Eukaryotes. The diversity of eukaryotes decreased at thermophilic phase, in addition dominant bands in mesophilic phase were decreased in the intensity. The number of DGGE bands increased at cooling and maturing phases where some selected bands characterized as Ascomycota, Basidiomycota, Zygomycota, Gymnamoeba, Chlorophyta and Arthropods.
Domestic waste, Composting, 18S rRNA, DGGE analysis
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