ISSN: 0973-7510

E-ISSN: 2581-690X

N.A. Ghanwate and P.V. Thakare
Department of Microbiology, Sant Gadge Baba Amravati University, Amravati – 444 602, India.
J Pure Appl Microbiol. 2012;6(2):875-879
© The Author(s). 2012
Received: 15/10/2011 | Accepted: 29/11/2011 | Published: 30/06/2012
Abstract

The chronic nature of some urinary tract infections (UTI) is being attributed to the ability of E. coli to form a biofilm. Bacteria growing within a biofilm lose their sensitivity to antibiotic quickly, thus resulting in persistent infections. The uropathogenic E. coli isolated from 200 samples were tested for antimicrobial susceptibility by disc diffusion method. In vitro quantitative estimation of biofilm formation by the isolates was determined by tube method. Comparison of two different media, for biofilm formation i.e. Luria Bertani broth(LB) and Brain Heart Infusion(BHI) broth with and without 2% sucrose were studied. From 77 E. coli isolates, 40 were positive for in vitro biofilm production. Among them 11 were classified as strong biofilm producers and 29 as moderate. It was found that 52% of biofilm producing E. coli were resistant to amoxicillin, 49% to cotrimoxazole, 41% to norfloxacin, 40 % to gentamycin and nalidixic acid, 35% to chloramphenicol and 31% to ciprofloxacin. The percentage of resistance in the nonbiofilm producing E. coli isolates ranged between 6-31%. Comparison of two media showed that the percentage of biofilm formation was more in LB than in BHI broth. There was a significant correlation between biofilm production and antibiotic resistance. The present study demonstrated that uropathogenic E.coli have high propensity to form biofilm that renders it resistant to conventional antimicrobial therapy.

Keywords

E. coli, Biofilm, UTI, Antibiotic resistance

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© The Author(s) 2012. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.