ISSN: 0973-7510
E-ISSN: 2581-690X
The b-galactosidase producing fungus Aspergillus flavus was isolated from marine samples, in our laboratory was used for strain improvement by physical and chemical methods. A wild isolate of Aspergillus flavus, was subjected to mutagenic treatment, by ultraviolet light and nitrous acid. Survival curves were obtained and mutants were selected using different mutagenic doses which gave 1 to 5% rate of survival. The mutated (A. flavus) strains of UV1 and UV11 were selected after the UV treatment. These two strains UV1 and UV11 were again treated with nitrous acid for further improvement of â-galactosidase yield. After physical and chemical mutation, 2.16 times increase in the production of intracellular b-galactosidase and 1.58 times increase in the extracellular b-galactosidase was obtained.
β-galactosidase, Aspergillus flavus, Nitrous acid treatment, UV treatment
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