Herpes simplex virus type 1 (HSV-1) is a common infectious agent worldwide. It can cause earnest illnesses at each stage of life. Data on seroprevalence of HSV-1 antibody (Ab) in Iraqi population is limited. This study aimed on determination the frequency of HSV-1 IgG Ab in apparently healthy blood donor of Baghdad province, capital of Iraq. In this study, serum samples were collected from 454 apparently healthy blood donor (385 male and 69 female), their ages ranged from 18 to 64 year. This study was conducted through the period from October, 2018 to February, 2019. Demographic data were gathered by a well-designed questionnaire. Five (5) ml of blood sample was collected and centrifuged, all obtained sera samples were tested using Enzyme linked Immunosorbent Assay (ELISA) for determine of HSV-1 IgG Ab. Totally, HSV-1 IgG Ab were positive in 427 out of 454 (94.1%) among studied population. The age groups of (21-30) years and (31-40) years had the highest percentage of seropositivity for anti HSV-1 IgG Ab (39.1% and 32.3%, respectively), while the lowest seropositivity was found in age groups (>50) years and  (£20) years (4.4% and 5.4%, respectively). The frequency of HSV-1 IgG Ab was higher among male (84.8%) compared with females (15.2%), however, it was not statistically significant (P > 0.05). Results confirm a high frequency of anti HSV-1 IgG Ab among Iraqi population. The high frequency of HSV-1 infection underlines the need for focusing on preventive efforts and education among the population about HSV-1 and its complications.