ISSN: 0973-7510

E-ISSN: 2581-690X

M. Ashwini1 , Archana1, Shamarao Jahagirdar2, G.S. Geeta1 and Smita R. Babar1
1AICRP on RES (Bioconversion Technology), Main Agriculture Research Station, University of Agricultural Sciences, Dharwad – 580 005, India.
2Department of Plant Pathology, AICRP on Soybean, University of Agricultural Sciences, Dharwad – 580 005,India
J Pure Appl Microbiol. 2015;9(2):1295-1299
© The Author(s). 2015
Received: 13/02/2015 | Accepted: 11/03/2015 | Published: 30/06/2015
Abstract

In the preliminary study, three hundred and seventy six Lactic Acid Bacterial (LAB) isolates obtained from the various natural sources were morphologically characterized and screened against the fungal pathogen Colletotrichum musae, causal agent of anthracnose of banana. The dual culture assay was employed to determine in vitro antagonistic activity of the LAB isolates. Among these twenty four, LAB isolates inhibited the mycelial growth of the pathogen. Out of twenty four, only two isolates viz. LS-36 and LS-44 exhibited maximum zone of inhibition. Based on 16S rRNA gene sequencing, LS-36 and LS-44 revealed maximum similarity to Lactobacillus brevis.

Keywords

Biocontrol, Lactic acid bacteria, Anthracnose, Colletotrichum musae

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