ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Lekha Singh1,2, Gaurav Sharma1  , Gyanendra Awasthi2, Lokendra Kumar2,3, Mohammad Irfan Ali1 and Sarmad Moin1
1School of Applied Sciences, Suresh Gyan Vihar University, Jaipur – 302 033, India.
2Department of Biochemistry, Dolphin PG Institute of Biomedical and Natural Science, Dehradun – 248 007, India.
3Department of Microbiology, PM College of Education Karsua, Aligarh – 202 001, India.
J Pure Appl Microbiol, 2019, 13 (3): 1855-1861 | Article Number: 5852
https://doi.org/10.22207/JPAM.13.3.63 | © The Author(s). 2019
Received: 03/09/2019 | Accepted: 24/09/2019 | Published: 30/09/2019
Abstract

Rhodococcus sp. LKE-021 from soil samples of the region of Gangotri (10,000 feet of average height) of Uttarakhand Himalayas, India, produced a thermophilic esterase. The physiological and morphological characteristics of the isolated Rhodococcus sp. LKE-021 detected as Gram Positive, rod shape, catalase positive, indole negative, positive to glucose and xylose fermentation test, and can grow on the Nutrient Broth medium. Esterase production confirmed on the basis of spectrophotometric enzyme assay. Taxonomic characteristics Rhodococcus confirmed by 16s rRNA gene sequencing.

Keywords

Esterase, hydrolytic enzymes, Rhodococcus, LKE -021, extremophiles enzyme, thermophilic enzyme.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.