ISSN: 0973-7510
E-ISSN: 2581-690X
The main purpose of the work was to screen intracellular lipase-producing microbial with high selective synthesis of 1,3-diolein, a healthy lipid. The screening model is a combination of Rhodamine B-olive oil agar plat, para-nitrophenyl palmitate (r-NPP) for ester synthetase activity and HPLC analysis of products of glycerolysis of triolein. The results show that the strain GZUF36 (CCTCC No. M2012538) can be used to catalyze the selective synthesis of 1,3-diolein. 1,3-Diolein yield is 11.12% (w/w) by whole-cell of GZUF36 catalyzed glycerolysis and it is accounted for 72% of total diolein (including 1,3- and 1,2-isomer). Then, GZUF36 was identified as Aspergillus niger by morphology and 18S rDNA sequence analysis. It is also suggested that the model can be applied to screen other lipases with high ester synthetase activities.
Aspergillus niger, 1,3-diolein, Identification, Intracellular lipase, Screening
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