ISSN: 0973-7510
E-ISSN: 2581-690X
20 fungal strains were screened from alkaline soils such as black cotton soil, groundnut field, milk processing unit, Kotappakonda hill area in Guntur Dist and Tirumala in Chittur District, Andhrapradesh, India. KS2 exhibited the prominent zone of clearance on modified seed medium at pH 9.0. The isolated species were identified basing on colony morphology,microscopic morphology,growth presence on temperature and pH of test fungal organism were identified as Aspergillus flavus. The alkalophillic isolate Aspergillus flavus shows maximum alkaline protease enzyme activity was (2000 U/mL) at pH 8.5, 40°C for 72 hr. These characteristics its potential use in alkaline protease production.
Alkaline protease, isolation, screening, Aspergillus flavus
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