ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
S.M.N. Moustafa1,2
1Biology Department, College of Science, Jouf University, P.O. Box: 2014, Sakaka, Saudi Arabia.
2Department of Botany & Microbiology, Faculty of Science, Minia University, El-Minia, Egypt.
J Pure Appl Microbiol. 2018;12(3):1525-1535
https://doi.org/10.22207/JPAM.12.3.57 | © The Author(s). 2018
Received: 10/07/2018 | Accepted: 05/09/2018 | Published: 30/09/2018
Abstract

This study was designed to identify some fungi responsible for damage of fruits in storage and sale outlet markets.  Results declared the role of CMC-ase and poly galacturonase (PG) enzymes secreted by three fungi of  Alternaria alternata, Penicillium italicum and Rhizopus stolonifer in decomposition of tomato, lemon and cucumber fruits at the grocery markets in Sakaka, Saudi Arabia.  CMC-ase and PG were produced after 2 days for  P. italicum, R.  stolonifer whereas very little quantity was detected for A. alternata.  Maximum CMC-ase and PG production were seen after 8-10 days of incubation at 28ºC.  Enzyme production started at a temperature of 5°C but in small quantities, then the production began to increase steadily until the temperature of 28°C, which produced the highest amount of enzymes. The production began to decline starting at 35ºC to be at the lowest rates at 40ºC.  Enzymes were produced at pH values of 4.5-9.5, with optima between 5.5-6.5 for the three tested fungi.  Acidic medium of pH 4.5, and alkaline medium (above pH 6.5) affected negatively on the production of enzymes, and this effect was  more evident in the exo CMC-ase and exo PG than in endo enzymes. Results showed that the mixture of menthol crystals & Eucalyptus oil & camphor & methyl salicylate oil-rich air inhibited growth of R. stolonifer and A. alternata by 100% and inhibited the growth of P. italicum by approximately 50%, compared to the control sample.  Camphor and mint oils gave negative effect.  Application of devices to emit the odor and quench this mixture of menthol crystals & Eucalyptus oil & camphor & methyl salicylate to fumigate the air of stores and outlets for selling vegetables and fruits are recommended.

Keywords

Alternaria alternata, Enzymes, Essential oil, Penicillium italicum, Rhizopus stolonifer, Rotted Fruits

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© The Author(s) 2018. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.