Review Separation and Purification of Proteins with Chromatography Applications

ISSN: 0973-7510

E-ISSN: 2581-690X

Huizhi Zhang¹, Daidi Fan¹ , Jianjun Deng¹, Xiaoxuan Ma¹, Chenghui Zhu¹ and Wenjiao Xue²

¹Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi’an, Shaanxi, China.

²Shaanxi Provincial Institute of Microbiology, No. 76 Xiying Road, Xi’an – 710 043, China.

J Pure Appl Microbiol. 2013;7(Spl. Edn.: April):07-12

Received: 03/03/2013 | Accepted: 14/04/2013 | Published: 30/04/2013

Abstract

The separation and purification of recombinant proteins are an important issue on bioprocessing. It not only contacts our lives, but associates with genetic engineering and expression. Because it is a junction of chemistry and biology, researchers should find a method to have a common effect on them for the separation and purification of protein. Though chromatography technology develops very quickly, it is difficult to meet requires of the separation and purification of protein. Despite these difficulties, chromatography, having been updated to solve the separation and purification of proteins constantly, which have been found widespread applications in the large scale purification of proteins from mammalian cells and microbial feedstocks in industrial bioprocessing. And others methodologies also are studied on the separation and purification of proteins.

Keywords

Review, protein, Chromatography, Bioprocessing

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