The human oral environment allows the growth of various microorganisms like staphylococcus aureus; streptococcus; enterococcus; klebsiella; e.coli; pseudomonas; candida albicans. As the colonization progress the pathogens start affecting the soft tissues of oral cavity. The maintainance of dentures plays a key role in preventing the pathogens to affect the oral cavity. The aim of the study is to identify the most common pathogens that invades the surface of the denture and to eradicate the pathogens. A study was conducted among 30 geriatric patients attending the OPD of saveetha dental college, saveetha university. 20 denture blocks were prepared with a size of 5x5mm of which 10 were impregnated with an antibiotic agent amoxicillin 500mg and another 10 were impregnated with an antifungal agent fluconazole 150mg. The blocks were reevaluated for the degree of growth of microbial pathogens. The results reveals that the microbial growth medium inoculated with the swab samples collected from 30 different subjects shows ballooning of S.Aureus and C.Albicans. Fluconazole impregnated blocks show the decreased thickness of candidal growth whereas, Amoxicillin impregnated blocks shows the clear zone of inhibition of bacterial growth and the average clear zone is determined. As the candida infections are concerned, fluconazole is effective as expected in control of bacterial growth. The amoxicillin shows the marked effect against the bacteria. The study gave a scope for further investigations with incorporating newer agents or different agents at various concentrations.