ISSN: 0973-7510

E-ISSN: 2581-690X

Z. P. Bhathena and Sachin S.Tawde
1Department of Microbiology, Bhavan’s College, Andheri-(W), Mumbai – 400 058, India.
J Pure Appl Microbiol. 2014;8(5):4189-4194
© The Author(s). 2014
Received: 28/04/2014 | Accepted: 15/06/2014 | Published: 31/10/2014
Abstract

A thermophilic alkalophilic organism isolated from the salt pan bed was used for the production of alkaline protease enzyme. The enzyme purification was carried out by using salt precipitation and DEAE cellulose chromatography to yield enzyme with a  specific activity of the 225U/mg .The enzyme had a  pH and temperature optima of 9.0 and 550C respectively and was capable of  recovering silver from exposed X-ray film at a recovery efficiency of 84.5%.. The recovered silver exhibited 98.10% purity which was better than the 70.5% recovery rate and 97.83% purity of silver recovered by traditional chemical method

Keywords

X ray films ,thermoalkalophilic, protease, extraction, Silver

Article Metrics

Article View: 919

Share This Article

© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.