qPCR detection of Rhodobacter sphaeroides Phage in the CoQ10 Production

ISSN: 0973-7510

E-ISSN: 2581-690X

qPCR detection of Rhodobacter sphaeroides Phage in the CoQ¹⁰Production

Kejun Li¹ , Haina Wang² and Ruiping Zhang¹

¹Lab of Space Microbiology, Center of Space Biology Research, China Academy of Space Technology, Peking, China.

²Institute of Microbiology, Chinese Academy of Science, Peking, China.

J Pure Appl Microbiol. 2015;9(3):1793-1796

Received: 26/04/2015 | Accepted: 05/06/2015 | Published: 30/09/2015

Abstract

Coenzyme Q₁₀is industrially produced by fermentation and extraction from Rhodobacter sphaeroides. However, phage pollution is a fatal phenomenon in the fermentation industry. The infection of R. sphaeroides by phase was reported for the first time in this study. Based on the obtained genetic sequence from the phage, a quantitative polymerase chain reaction (qPCR) phage detection profile was developed for early detection during the pilot culture to prevent phage pollution in the production lines.

Keywords

Rhodobacter sphaeroides, phage pollution, phage detection, qPCR

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